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Before starting, it helps to be clear about the purpose of the protocol

Sensitivity of serum methylmalonic acid and total homocysteine determinations for diagnosing cobalamin and folate deficiencies

Though landmark technique is well described in literature, the whole course of the LFCN as well as its formation is highly variable among individuals

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It is essential for the formation of red blood cells, proper neurological function, and DNA synthesis

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