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Methods 21 , 14301443 (2024)

The maturation process was discovered to be accelerated by improving the mitochondrial health of oocytes

Archived from the original on 16 July 2021

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Electrophysiology The composition of the solutions is detailed below: NMDG cutting solution (135 mM NMDG, 1 mM KCl, 1.2 mM KH2PO4, 1.5 mM MgCl2, 0.5 mM CaCl2, 20 mM choline bicarbonate, and 10 mM glucose), ACSF (130 mM NaCl, 24 mM NaHCO3, 3.5 mM KCl, 1.25 mM NaH2PO4, 1 mM MgCl2, 2 mM CaCl2, and 10 mM glucose, aerated with 95% O2/5% CO2), and internal solution (108 mM Cs-Methane sulfonate, 4 mM MgCl2, 1 mM EGTA, 9 mM HEPES, 5 mM Mg-ATP, 15 mM phosphocreatine (di-) TRIS, 1 mM Na-GTP, and 5 mM QX-314, pH 7.4, 280 mOsm)