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However, deletion of MLKL or inhibition of RIPK1 in macrophages does not completely rescue Mtb-infected cells from death, 84,101 suggesting that, although the deficiency of MLKL or RIPK1 can abrogate the canonical necroptosis pathway, upstream TNF/TNFR1-mediated signaling may stimulate the induction of other cell death pathways during Mtb infection

Heil et al

10.1002/kjm2.12684 77 LantujoulS.SalameireD.SalonC.BrambillaE

IL-1 activates IB kinase (IKK), and subsequently, the IKK complex activates NF-B by phosphorylating the IB molecule, leading to its degradation and the release of NF-B (171)

One microgram of total RNA for each sample was reversely transcribed into cDNA by a commercial kit (RR047A, Takara Bio, Japan) according to the manufacturers protocol