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After 16 h of incubation, the cells were collected, washed with PBS, and stained with 2,7-dichlorodihydrofluorescein diacetate (10 M, diluted in serum-free medium) at 37 C for 30 min

Increased chemiluminescence was used to visualize Immuno-reactive bands

Over time, factors such as age, lifestyle, genetics, previous injury, inflammation and biomechanical instability can lead to the breakdown of collagen and slow the process of healthy collagen synthesis

it can provide significant therapeutic benefits , especially for individuals with problematic menstrual symptoms

10.1186/s10020-024-00824-9 235 YousefH.KhandokerA

Ultra-low dose interleukin-2 promotes immune-modulating function of regulatory t cells and natural killer cells in healthy volunteers