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This uptake results in aggregation of lipoylated TCA cycle enzymes, particularly pyruvate dehydrogenase complexes (dihydrolipoamide S-acetyltransferase (DLAT), pyruvate dehydrogenase E1 subunit 1 (PDHA1), and pyruvate dehydrogenase subunit (PDHB)), as direct Cu-binding targets, accompanied by loss of iron-sulfur cluster proteins (77)

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For control and test RNA, library construction was performed using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions