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Proteins were transferred to 0.2 m nitrocellulose membranes using Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, 1704150)

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Unsaturated fatty acid moieties of membrane lipids are attacked by free radicals, which leads to a self-replicating chain reaction of non-enzymatic lipid peroxidation that produces malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), isoprostains and other compounds

One promising strategy involves modulation of activating transcription factor 6 (ATF6), a key mediator of adaptive UPR signaling

Through reduction in beneficial SCFAs, gut dysbiosis impares gut function and contributing to systemic inflammation and oxidative stress

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