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The resulting cells were fixed in 4% paraformaldehyde and incubated with a blocking buffer for 1 h, and the cells were permeabilized with 0.2% Triton and 0.1% Tween 20 in a blocking buffer (3% bovine serum albumin in PBS) for 2 h

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For example, endovascular Tissue Factor (TF), which is a pro-coagulant protein, was found to be 10 x higher in severe COVID-19 patients (median = 231 fM) compared to moderate disease severity (median = 25 fM) (151)

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