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For the non-interventional cohort, a group size of 20 subjects will detect a minimum difference in baseline GSH of at least 16% between young and elderly healthy subjects (0.05 alpha, 0.8 power) based on an inter-subject CV of 22%

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In addition, the investigators discovered that melittin reduced carnitines, polyamines, adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide (NAD+) (Erkoc et al., 2022)

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Epidemiological data have consistently indicated that elevated levels of homocysteine in circulation are associated with an increased risk of cardiovascular disease (56, 57)