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Next, cells were incubated 1 hour with an anti-rabbit-PE conjugated antibody, stained with DAPI, mounted with Fluoreshield (Sigma) and photographed with a fluorescence microscope (ZEISS AxioVert A.1)

Learning Objectives By the end of this section, you will be able to: Explain the importance of water in the body Contrast the composition of the intracellular fluid with that of the extracellular fluid Explain the importance of protein channels in the movement of solutes Identify the causes and symptoms of edema The chemical reactions of life take place in aqueous solutions

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Formation monitors these developments closely and adjusts its protocols in accordance with current federal and state regulations