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Description

A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR

Bertorelle, F

Ulker S, McKeown PP, Bayraktutan U

Beads were repeatedly washed with GST-lysis buffer, prepared for SDS-PAGE, and finally analyzed by Western blot using antibodies against GFP, mRFP, and Flag-tag when needed, as well as HRP-conjugated secondary antibodies for ECL detection