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Cells were incubated with a mouse monoclonal antibody targeting the human transferrin receptor (Clone H68.4, #136800, Invitrogen, Eugene, OR) diluted to a final concentration of 2 g/mL in PBS overnight at 4 C

EAD provided rich MS/MS spectra, enabling the identification of an N-dealkylated midazolam N-glucuronide metabolite

This molecular characteristic provides a basis for the development of subtype-specific treatment strategies (164, 165)

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Meanwhile, the total antioxidant capacity (T-AOC) also increased, as confirmed by the evidence of the superior ROS scavenging by APP-treated serum