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This process yielded 25.0 mg of speciofoline (95% purity based on UPLC-UV), the structure of which was solved by comparison of the calculated ([M + H] + C 22 H 29 N 2 O 5 , 401.2076) and measured (401.2067) m/z values and by comparison of key 1 H-NMR signals (Figure S1 and Table S2) and the ECD spectra (Figure S2) with those reported in the literature 25

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