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Description

Opportunities and challenges in phenotypic drug discovery: An industry perspective

doi: 10.1038/nsmb.3460 76 CunninghamCCDeChateletLRSpachPIParceJWThomasMJLeesCJet al

To link these changes to functional adaptations, we selected key nutrient transporters and metabolic enzymes for single-cell validation by spectral flow cytometry, focusing on molecules involved in amino acid uptake, glycolysis and the pentose phosphate pathway 24,25

Aust Endod J

Unresolved questions and future directions Currently, despite promising prospects in preclinical models, targeting glutamine metabolism faces challenges in clinical trials due to inherent heterogeneity in glutamine metabolism within tumor cells and immune cells, as well as its complex impact on immune responses