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Hepatic-specific Zip8 KO mice showed significantly lower hepatic Mn levels (Powers et al., 2023), as did Zip8 loss-of-function mutations (Sunuwar et al., 2020), while bile Mn levels were increased by two-fold (Sunuwar et al., 2020)

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The plates were washed with PBS-Tween 0.05% and blocked with PBS-4% bovine serum albumin (BSA, Sigma-Aldrich)