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To detect any contamination by genomic DNA, the primers were designed to span intron-exon boundaries

The pathophysiology of elevated vitamin B12 in clinical practice Significance of elevated cobalamin (vitamin B12) levels in blood Elevated levels of serum cobalamin may be a sign of a serious, even life-threatening, disease

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Based on a review retrieved from PubMed, it is one of the major chemical forms of B12 obtained from the diet, alongside methylcobalamin and adenosylcobalamin, and it sits at the centre of how cells take up and route the vitamin (Hannibal & Jacobsen, 2022, DOI)