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In addition to endogenous biomarker triggers, external physical cues such as near-infrared light, ultrasound, or magnetic hyperthermia can be exploited as responsive switches to achieve on-demand and site-specific release of copper and iron ions

doi:10.1097/scs.0000000000001880 141

In thin-layer chromatography (TLC), the analytical tool extensively used because of its easiness, relative cheapness and high sensitivity, the compounds may be separated quickly by planar chromatography on silica, cellulose, polyamide or chemically- modified plates, and detected, either directly or after reacting with a specified reagent ( Genetic analyses represent are a new and powerful tool for the reliable identification of a given species, even in a mixture, Allowing to identify traces of DNA in liquid and dry extracts also after repeated processes of dilution/concentration/separation (Novak et al., 2007)

Samples were then washed two times with cold PBS and read using AttuneTM Cytometer (Thermo Fisher Scientific)

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